[Protein-analysis] zinc finger protein purification issue

김종문 via proteins%40net.bio.net (by jin.zongwen from gmail.com)
Fri Jan 15 01:33:25 EST 2010

I recently started to purify a protein containing zinc finger modif.

the protein consist of flexible linker and his tag in the N term and C
terminal of the mofit.

the Zinc finger itself is consisted from three zinc finger motifs.

It expressed well in the E.coli, and I was able to purify the protein, but
when I tried to concentrate it by using a membrane filter( amicon centricon)
the protein start to aggregate.

what is more a concern is that gentle swiring in 4'C would generate cotton
like aggregates in the tube.

I would like to ask if anyone have expperience on zinc finger protein,
expecially small zinc finger proteins like this one.(15kDa)

any suggestion are welcomed.

Best regards to every one

Zongwen Jin
Biomolecular Engineering Lab
Dept. of Biological Sciences, KAIST
335 Gwahangno, Guseng-dong, 305-701
Yuseong-gu, Daejeon, Republic of Korea
Researcher ID: B-4590-2009
Lab: +82-42-350-2656
Mobile: +82-10-7460-9518
E-mail: sutem from kaist.ac.kr
K-Internet : 070-8288--0606

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