[Protein-analysis] Re: Protein precipitation in HPLC buffer

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Fri Jan 23 13:25:12 EST 2009

Am 22.01.2009, 08:28 Uhr, schrieb Mouldi SAIDI <m.saidi from cnstn.rnrt.tn>:

> I would like to separate caseins using HPLC and it is known that  
> acetonitrile precipitates proteins. Is it possible to use it

Sure. However, proteins - as opposed to small peptides - can not be  
separated by reverse phase chromatography (with a C18 column and  
acetonitrile gradient). You need other chromatographic principles. I have  
never worked with caseins, but consider ion exchange chromatography,  
chromatofocussing or hydrophobic interaction chromatography. A literature  
search on scholar.google.com may be a good first step.

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