My project is protein extraction and identification of Curculigo latifolia
fruit(somehow protein profiling). i already extracted all the proteins
through TCA-aceton methanol washes phenol precipitation. and i plan to do
two dimensional gel electrophoresis to get spots and then send the spots to
Ms/MS to sequence, but it would be very expensive to apply for all spots.So
would you tell me if you were me, which kind of spots you would choose?
please help me narrow my identification.