Hello,
It varies from protein to protein, but I do not have experience
with fibronectin or streptavidin. It's usually better to freeze
proteins with glycerol, at higher concentration, and as quickly as
possible (in pcr tubes plunged into liquid nitrogen).
ho
> Hello
>> (please excuse any bad english)
>> I'm a nanotecnology student with a molecular background but without much
> hands-on in that field.
>> I've ordered and received a couple potions of proteins and would like to
> know some general handling of these.
>> Proteins comes either lyophilizised or in solution at ca 5C.
> Is it normally okay to freeze the proteins again to keep their life-time
> up even if they come in solution and does it depend on the protein?
> In my case it is streptavidin and human plasma fibronectin.
>> I'm guessing it's okay to freeze them in diluted aliquots even if they
> come in solution and no matter if they are in salt or salt-free, and
> then thaw them just before use. My supervisor is a bit sceptic though.
>> Thank you for any help
