[Protein-analysis] exponential phase in M9 (or, when to induce)

assa sittner via proteins%40net.bio.net (by sittner from lkb.ens.fr)
Fri Oct 17 03:58:39 EST 2008

Hi everyone,
I am planning on growing E.coli (rosetta), in M9 medium:
M9 salts (http://openwetware.org/wiki/M9_salts)+
+carbon sources (glycerol 1%, casamino acids 0.1%)
+kanamycin (vector)
+chloramphenicol (pRARE)

agitation ~ 200rpm
could anyone give me an rough approximation for:
1. the OD in mid-exponential phase (or, when i should induce)?
2. how long should it take to get there?



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