I am planning on growing E.coli (rosetta), in M9 medium:
M9 salts (http://openwetware.org/wiki/M9_salts)+
+carbon sources (glycerol 1%, casamino acids 0.1%)
agitation ~ 200rpm
could anyone give me an rough approximation for:
1. the OD in mid-exponential phase (or, when i should induce)?
2. how long should it take to get there?