DK wrote:
> In article <mailman.209.1222814664.29717.proteins from net.bio.net>, "Fasulo, Lisa M" <lisa.m.fasulo from pfizer.com> wrote:
>> I am trying to calculate the specific activity of my [3H]-ligand but
>> don't know how to do it. The package insert for my radioligand states
>> that the Specific Activity is 200 mCi/g but I need to know what the
>> Specific Activity is in Ci/mmol. How do I calculate this? The
>> molecular weight of the radioligand is 463.6 g/mol. Can anyone walk me
>> through the calculations to give the specific activity in Ci/mmol? The
>> only other info I have is the concentration of the radioligand which is
>> 1mCi/mL.
>> The answer is 10.785 Ci/mmol and the concentration of radioactive
> compound is 92.72 mM.
>> This is very basic, Lisa. Do something urgent to figure it out
> yourself. Or do yourself and your company a favour and quit.
> It is THAT important!
>> Hope the hint above helps.
>>Actually, the SA is 92.72 _milli_Curies/mmol and the concentration is
10.785 mM (to probably more significant figures than it deserves).
.2 Ci/g x 463.6 g/mol = 92.72 Ci/mol = 92.72 mCi/mmol
(1 mCi/ml)/(92.72 mCi/mmol) = .010875 mmol/ml = 10.875 mmol/liter
Not sure which people need to do everyone a favor and quit. Yeesh...
