Am 04.10.2008, 07:57 Uhr, schrieb jh <biocjh from gmail.com>:
> Dear all,
>> I stained a gel with PAS(periodic acid stain) method for visualizing
> glycoproteins.
> However, even protein markers and the reconstructed protein expressed
> by E. coli could be stained.
> Whether the PAS method is specific for visualizing glycoproteins?
> And how to explain the non-glycoprotein can also be stained?
>> I am appreciated of any help of you!
Many marker proteins are glycosylated, this is true for almost all
proteins that are secreted.
Since you did not say how you did the staining here a reference that I
know works:
Segrest,J.P. & Jackson,R.L.: Molecular Weight Determination of
Glycoproteins by Polyacrylamide Gel Electrophoresis in Sodium Dodecyl
Sulfate
Meth. Enzymol. 28 (1972) 54-63
G. Fairbanks, T.L. Steck & D.F.H. Wallach: Electrophoretic analysis of the
major polypeptides of the human erythrocyte membrane
Biochemistry 10 (1971) 2606-2617
Btw: It is much more convenient to do the PAS-staining on a western blot
than in the gel, the incubation times are shorter and less reagent is
required.
