[Protein-analysis] Re: on PAS for glycoprotein

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Tue Oct 14 15:30:14 EST 2008

Am 04.10.2008, 07:57 Uhr, schrieb jh <biocjh from gmail.com>:

> Dear all,
> I stained a gel with PAS(periodic acid stain) method for visualizing
> glycoproteins.
> However, even protein markers and the reconstructed protein expressed
> by E. coli could be stained.
> Whether the PAS method is specific for visualizing glycoproteins?
> And how to explain the non-glycoprotein can also be stained?
> I am appreciated of any help of you!

Many marker proteins are glycosylated, this is true for almost all  
proteins that are secreted.

Since you did not say how you did the staining here a reference that I  
know works:

Segrest,J.P. & Jackson,R.L.: Molecular Weight Determination of  
Glycoproteins by Polyacrylamide Gel Electrophoresis in Sodium Dodecyl  
Meth. Enzymol. 28 (1972) 54-63

G. Fairbanks, T.L. Steck & D.F.H. Wallach: Electrophoretic analysis of the  
major polypeptides of the human erythrocyte membrane
Biochemistry 10 (1971) 2606-2617

Btw: It is much more convenient to do the PAS-staining on a western blot  
than in the gel, the incubation times are shorter and less reagent is  

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