[Protein-analysis] RE: Calculations for specific activity

Morales, Victor via proteins%40net.bio.net (by Victor_Morales from URMC.Rochester.edu)
Thu Oct 2 08:17:34 EST 2008


Specific activity (mCi/g) X molecular weight (g/mol) = mCi/mole   ----->
200 mCi/g X 463.6 g/mol = 92720 mCi/mol

If you need it in mCi/mmole:

Specific activity (mCi/g) X molecular weight (g/mol) X 1 mol
_____________________________________________________________   =
                          1000 mmol

200 mCi/g X 463.6 g/mol X 1 mole
_____________________________________ =  92.72 mCi/mmole 
               1000 mmoles

Hope this helps,
Victor Morales

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Today's Topics:

   1. Help with calculations for specific activity  (Fasulo, Lisa M)


Message: 1
Date: Tue, 30 Sep 2008 16:55:26 -0400
From: "Fasulo, Lisa M" <lisa.m.fasulo from pfizer.com>
Subject: [Protein-analysis] Help with calculations for specific
To: <proteins from magpie.bio.indiana.edu>
<3F4063B77CC1F445933078B93A0A0C720855F3CF from groamrexm05.amer.pfizer.com>
Content-Type: text/plain;	charset="us-ascii"

I am trying to calculate the specific activity of my [3H]-ligand but
don't know how to do it.  The package insert for my radioligand states
that the Specific Activity is 200 mCi/g but I need to know what the
Specific Activity is in Ci/mmol.  How do I calculate this?  The
molecular weight of the radioligand is 463.6 g/mol.  Can anyone walk me
through the calculations to give the specific activity in Ci/mmol?  The
only other info I have is the concentration of the radioligand which is



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