If a cold temperature is important maybe you could just run the Speedvac
in a cold room (with the heater turned off of course).
Allison
Dorit Grunberger wrote:
> Saw this online and thought to ask you if it makes sense to hook up a
> speedvac to a lyophilizer both as source of vacuum and no less important, a
> cold environment to keep my extract happy. I¹m doing chloroform : methanol :
> water extracts of small tissue samples. The total volumes for extraction
> will be small and I was hoping to use a speedvac to dry/concentrate my
> MeOH/H2O phase. It¹s critical for the sample to remain as cold as possible,
> since the analysis of contents is done by NMR and we¹re hoping to maintain
> integrity of some very easily degradable metabolites (phospho-choline for
> example).
>> Bottom line: do you think this will work?
>> Thanks in advance for any help.
>> Dorit Grunberger
> Department of Radiology
> email: dorit.grunberger from radiology.ucsf.edu> 415.514.4845 office
> 415.385.0977 cell
> 415.514.2550 fax
>>>
