Saw this online and thought to ask you if it makes sense to hook up a
speedvac to a lyophilizer both as source of vacuum and no less important, a
cold environment to keep my extract happy. I¹m doing chloroform : methanol :
water extracts of small tissue samples. The total volumes for extraction
will be small and I was hoping to use a speedvac to dry/concentrate my
MeOH/H2O phase. It¹s critical for the sample to remain as cold as possible,
since the analysis of contents is done by NMR and we¹re hoping to maintain
integrity of some very easily degradable metabolites (phospho-choline for
example).
Bottom line: do you think this will work?
Thanks in advance for any help.
Dorit Grunberger
Department of Radiology
email: dorit.grunberger from radiology.ucsf.edu
415.514.4845 office
415.385.0977 cell
415.514.2550 fax