[Protein-analysis] Re: protein extraction

[Dr Engelbert Buxbaum]

Am 14.01.2008, 10:17 Uhr, schrieb kyoli <allicindixvin from gmail.com>:

> i am performing 1-D gel electrophoresis for protein  from leave but
> chlorophyll is inhibiting for which i used acetone with tris. do
> anyone have any method to extract protein in this combination without
> using liquid nitrogen

Chloroform/Methanol precipitation should work

@article{Wes-84,
         AUTHOR= {D. Wessel and U.I. Flügge},
         TITLE= {A Method for the quantitative recovery of protein in the  
presence of detergents and lipids},
         JOURNAL= {Anal. Biochem.},
         VOLUME= {138},
         YEAR= {1984},
         PAGES= {141-143},
         LANGUAGE= {engl}
}