Hello
I´m performing western blots on lysates from EBV transformed lymphocytes. I
electrophorese the lysates on a 12% SDS PAGE and the incubation with the
anti p53 DO1 shows a double band in some individuals and only one band in
others, the difference between the bands is approx 3-4 kDa. This happens
without any treatment. Some one has experienced this???. Is this due to
phosphorylation???, p53 isoforms differentially expressed in different
individuals???.
I´ll appreciate any suggestion,
Alejandro
