[Protein-analysis] Re: Recombinant Protein precipitate out under 20 mM KPB/100 mM NaCl ???

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Mon Feb 18 16:37:39 EST 2008

Am 14.02.2008, 20:21 Uhr, schrieb Nico <brighttomorrow from gmail.com>:

> Under 20mM KPB/500mM NaCl, it was good. But when change to 20 mM KPB/100  
> mM NaCl,
> the protein precipitate out. What should I do? Change to Tris-HCl Buffer  
> or.....

Looks like your protein is an albumin, with elongated shape and asymetric  
charge distribution. At low ionic strength they form head-to-tail  
aggregates, which precipitate. You can either keep the ionic strength high  
or change the pH and hence the charges on the protein.

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net