Am 17.09.2007, 16:34 Uhr, schrieb Jyothi Jayaram
<jyothi.jayaram from gmail.com>:
> Hi there,
>> I need help in determining the concentration of the detergent Triton
> X-114
> in water saturated Triton 114. I know that once the detergent is water
> saturated, we have to determine the absorbance at 277 nm. After this, I
> really don't know how to correlate the value obtained to its
> concentration.
See Nag et al., Anal. Biochem. 237 (1996) 224-231 and Garewal Anal.
Biochem. 54 (1973) 319-324. The procedure works with all poly-oxethylen
detergents.
