Am trying to screen (slot blot) for mammalian expression of a secreted
recombinant His-tagged protein from serum containing
conditioned medium. Unfortunately the protein is undetectable using
any of the many protein specific antibodies tried so far, probably due
to some kind of binding with a component from the serum! I will
therefore have to go for a anti-His antibody and given I have never
been lucky in the past using this strategy (used Sigma anti-His HRP
conj.) I was wondering whether any of you may have any useful
suggestion.
Thanks
federico
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References
1. http://g.msn.com/8HMAEN/2749??PS=47575
