IUBio

[Protein-analysis] Re: Protien stucture MALDI

Isabelle Holdridge via proteins%40net.bio.net (by holdri39 from uwosh.edu)
Mon Nov 26 12:53:26 EST 2007



----- Original Message -----
From: proteins-request from oat.bio.indiana.edu
Date: Sunday, November 25, 2007 11:04 am
Subject: Proteins Digest, Vol 30, Issue 8
To: proteins from magpie.bio.indiana.edu

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> Today's Topics:
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>    1. Re: looking for a software to predict my protein
>       structure...! (DK)
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> 
> -----------------------------------------------------------------
> -----
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> Message: 1
> Date: Sat, 24 Nov 2007 06:59:11 GMT
> From: dk from no.email.thankstospam.net (DK)
> Subject: [Protein-analysis] Re: looking for a software to 
> predict my
> protein structure...!
> To: proteins from net.bio.net
> Message-ID: <3ZP1j.74$uc.5 from newsfe05.lga>
> 
> bazmohd from gmail.com wrote:
> >if any one can help...iam looking for a software or awebsite to
> >predict my protein structure...! iam working with a promissing
> >protein.
> 
> No one can help. There is no such thing. 
> Everyone hope there was but, alas, there isn't.
> 
> DK
> 
> If your protien is embedded in a gel you could try running on MALDI-TOF mass spec to identify and characterise your protien.  Once you determine a possible structure, then you can pay a company to make your protein based on the stucture you come up with from the mass spec.  Then test your protein against the created protein to see if they have the same function.  Our lab is trying to characterise proteins in this way.  We start with a silver stained 2-D gel to visualize the protein.  Then we will run cut that particular protein out of the gel and then run it through the MALDI-TOF mass spec.  It is very difficult, but supposidly it can be done.  Good Luck!

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