[Protein-analysis] Re: Glycerol during protein purification and protein oligomerization state

Frank Küster via proteins%40net.bio.net (by frank from kuesterei.ch)
Thu Jul 26 00:51:44 EST 2007

"Dr Engelbert Buxbaum" <engelbert_buxbaum from hotmail.com> wrote:

> I would however suspect that removal of the DNA could be the culprit:
> many  proteins become unstable if their ligand is removed. Perhaps
> adding short  DNA fragments, which are less likely to interfere with
> purification than  an entire bacterial chromosome, would help.

Or even just high concentrations (100 or 200 mM) of phosphate buffer.  I
know about an (unpublished) DNA-binding protein which can be triggered
to fold in a stopped-flow by mixing protein solution with  phosphate

Regards, Frank

(17:00:03) ***joeyh loves that install-info uses $'
(17:00:34) Yoe: what's $' again?
(17:00:49) joeyh: shorthand for make your perl program slow at the 
                  expense of readability

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