proteins-request,ÄúºÃ£¡
Usually, two many bands are caused by too much antibody you used. Tried to dilute more of your antibody when using. In my experience wet transfer has a much higher efficency than semi-dry tranfer. The only problem is buffer consuming and heat generating.
======= 2007-01-22 01:08:45 ÄúÔÚÀ´ÐÅÖÐдµÀ£º=======
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>>>Today's Topics:
>> 1. (no subject) (Manisha Mishra)
> 2. MicroCAL Origin 7.0 Software for ITC (Tyler Collins)
>>>----------------------------------------------------------------------
>>Message: 1
>Date: Thu, 18 Jan 2007 11:45:04 +0800
>From: "Manisha Mishra" <manishamishra1 At gmail.com>
>Subject: [Protein-analysis] (no subject)
>To: Proteins At magpie.bio.indiana.edu>Message-ID:
> <ce5117a50701171945y5070f352q3aeb1dd467350167 At mail.gmail.com>
>Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>>hi
>>I have one problem when i do western blot..
>>Sometimes i get single bands but many times i get so many bands..while my
>lab mates get few bands using the same antibody..What may be the probable
>reason????
>>also i want to know whether wet method is good for tranfer or semidry????
>>>--
>Regards
>Manisha Mishra
>>Manisha Mishra
>Molecular biology Unit
>Dr Klaus Heese
>SBS, NTU
>Singapore
>>>------------------------------
>>Message: 2
>Date: Fri, 19 Jan 2007 16:51:27 -0500
>From: "Tyler Collins" <collinst824 At duq.edu>
>Subject: [Protein-analysis] MicroCAL Origin 7.0 Software for ITC
>To: <proteins At magpie.bio.indiana.edu>
>Message-ID: <ECEGJIFPMLAOOGGDGKODKENPCCAA.collinst824 At duq.edu>
>Content-Type: text/plain; charset="iso-8859-1"
>>I am attempting to use our ITC MCS Unit from MicroCal to quantify the
>homodimer dissociation of a dimeric protein to monomer. Unfortunately,
>homodimeric species are difficult to analyze using the old Origin software.
>In addition, MicroCal will not provide a free upgrade in software to
>accommodate the models you want to use. Instead, they are charging $2500
>for software that includes a dimer dissociation model that would be useful
>to my work. Does anyone know where to get a possible bootleg version of the
>software, or do you have suggestions, books, articles that may aid in
>helping me write my own macro?
>>>------------------------------
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Sincerely,¡¡¡¡¡¡¡¡¡¡
Lu Falong
Group 808
Institute of Genetics and Developmental Biology, CAS
Beijing, PR China
FLLu At genetics.ac.cn
2007-01-22
