[Protein-analysis] (no subject)

guir0003 At umn.edu via proteins%40net.bio.net (by guir0003 At umn.edu)
Wed Jan 17 16:05:32 EST 2007

Hello all,

I am interested in immobilizing an IgG polyclonal antibody to a solid phase 
for affinity. I've tested the method with a commercially available 
hydrazide-functional, crosslinked agarose to be the support. Hydrazide 
should bind to aldehydes on periodate-treated glycoproteins, I'm told.

I tried to do that by periodate-treating a non-specific Rabbit IgG (RbIgG) 
and exposing it to the gel for coupling, then using a mouse anti 
rabbit~peroxidase conjugate as the target molecule, since that is easy to 
detect in fractions at low concentrations. I eluted with acetic acid pH 
~2.7, and neutralized the acidic fractions with Tris.

My problem emerges when the CarboLink gives me unacceptably high 
backgrounds, i.e., exposing it to RbIgG that has not been periodate-treated 
still apparently confers significant affinity for the target to the gel. 
Gel that has not been exposed to the RbIgG apparently does not bind the 

The peroxidase conjugate is detectable in low concentrations, and I haven't 
been able to account for all the activity that I measure in the solution 
that I load onto the columns. I know its protein concentration is far lower 
than it would be in practice, that is, even the molecule I'd be trying to 
purify should be more concentrated at that point than is the conjugate I've 
been using for practice.

Blocking the gel with BSA before exposing it to the RbIgG didn't seem to 
work either (:-P), any thoughts?

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net