use a desalting column to chnage the buffer instead of using dialysis.
but you may a chromatography system and a desalting coulmn...
"pradeep babu" <pradeepreddyj from gmail.com>
???????:mailman.1105.1170868316.19683.proteins from net.bio.net...
> Dear Sir,
> Iam glad to introduce myself as Pradeep from India, Iam working on
> recombinanat vaccine for Dengue, for that i need to do protein refolding,
> iam facing problem at the time of dialysis, protein is getting
> precipitated
> so please help me out if u have an idea,
>> tahnking you
>> Yours Sincerely
> Pradeep
> Junior Research Fellow
> India.
