[Protein-analysis] Re: Doing a western after coomassie staining

Luis M. Sanchez via proteins%40net.bio.net (by lmsp At uniovi.es)
Wed Feb 7 12:43:05 EST 2007

> Does anyone know a protocol to transfer protein after staining the gel
> with coomassie (10% Acetic acid and 50% EtOH, 0.2% Brilliant Blue) ?

You can check the article "Western Blot of Proteins from Coomassie-Stained  
Polyacrylamide Gels" by Ranganathan and De. It was published in Analytical  
Biochemistry 234, 102-104 (1996)

Luis M. Sánchez
Scientific-Technical Facilities
University of Oviedo
Campus del Cristo
Oviedo, 33006

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