Hello,
I am new to histological protocols. Recently, I completed an animal study
in which I harvested skin wounds and snap froze some of the samples
anticipating in situ hybridization studies to complement/confirm quantitative real time
RT-PCR. The tissue samples were stored at minus 20 (instead of minus 80)
and likely have degraded RNA.
My question: Is it possible to fix the previously frozen samples in
formalin and continue analysis via immunohistochemistry for the protein
(specifically proinflammatory cytokine) expression? Also, does minus 20 storage
(instead of minus 80) put the tissue at risk?
Thanks for your time.
Rich
************************************** Get a sneak peek of the all-new AOL at
http://discover.aol.com/memed/aolcom30tour
