[Protein-analysis] cross-linking

Максим Дюков mdyukov at mail.ru
Sun Sep 24 08:37:41 EST 2006

Hello everyone,

Could anyone tell me what type of unspecific homobifunctional protein cross-linker I could use that works at pH around 2. I'm planning to analyze quaternary structure using SDS-PAGE after cross-linking so the link should be stable under SDS-PAGE sample preparation conditions. My first thought was some amine-reactive cross-linker but I guess protonization blocks all conventional cross-linking reactions involving amines.
I will appreciate your suggestions.


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