Dear Dr. Gegenheimer,
I've read a post on the internet relating to the expression of toxic
proteins in bacteria. In this particular letter you wrote the following:
"A third method is to use a host cell lacking the T7 polymerase gene, and
induce target protein synthesis by infection with a lytic phage carrying the
T7 polymerase gene. The phage is available from Novagen and other sources."
If you have a chance, could you please let me know a bit more about this
particular system. For example, what strains lacking the T7 polymerase
have worked well for you, and is there a protocol associated to this
type of expression? Moreover, I assume that there is no need for
induction with IPTG since the induction would be enabled by the phage
polymerase. Does the expressed protein degrade in the media once the
bacteria have undergone phage-specific lysis?
Thank you very much for your attention to this matter.
Dept. of Horticulture