Dear All,
why sometimes the target protein size is bigger than the expected size when
running in SDS-PAGE? Some suggested that I should run my target protein
compared with un-prestained protein markers, or to make sure that there is
no cross-reaction once the immuboblot was performed. All of the above have
been tested but the results with bigger size of target protein than that of
expected one still a problem. Can anyone give me a clearer explaination or a
better way to verify or to confirm that I have the right target protein?
Thank you very much,
Kon Diew
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