[Protein-analysis] serine protease inhibition by plastic extractable material

rolando via proteins%40net.bio.net (by rolando.perdomo At infomed.sld.cu)
Sun Nov 19 19:19:55 EST 2006

Hi  Every one,

I found that during preparation of cellular extract to analyze serine
protease activity, if sample is sonicated in 1.5 ml eppendorf centrigue
tubes, the proteolytic activity is diminished. In other hand, if the
same cells are lysed by sonication using a glass reservoir no
inhibition appears. Does anyone know about relation of protease
inhibition and plastic-extractable material??? References????? Looking
forward to receive some light.

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