[Protein-analysis] Hydroxylamine cleavage

Greenshields, Anna Anna.Greenshields at nrc-cnrc.gc.ca
Thu May 11 12:27:25 EST 2006



I am trying to use hydroxylamine cleavage to cut a tag off a small
protein.  Within 3 hours of starting the cleavage rxn my protein ppts
(final conc, 2M HA, 4.8M guanidine, 20mM Ches).  The ppt seems to be
occuring before cleavage takes place.  I have tried adding different
concentrations of DTT and changing the pH of the reaction ( I usually
use pH9.5) but have not been able to inhibit the ppt of the protein.


Any suggestions? 




More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net