nightfirecat at gmail.com wrote:
> I think the detergent Brij-35 was involved in the precipitation.
>> The detergents always distroy the conformation of the proteins, and the
> unfolded or destroyed proteins are also proned to aggregation.
Not necessarily. If the protein in question is a trans-membrane protein
(which I suspect even though it was not stated) then detergents are
required to solubilise it out of the membrane and keep it in solution.
If the detergent concentration in such a preparation is lowerd below the
cmc, the mixed detergent/protein/lipid micells will loose the detergent
and the protein aggregates.
Brij-35 is a non-ionic detergent and has occasionally been used for
membrane protein isolation. However, in the cases where I tried it, it
yielded results much poorer than C12E8 or Triton-X100. But the protein
of the OP may behave differently.
