[Protein-analysis] Re: protein contamination with DNA???

kaj.stenberg at helsinki.fi.invalid kaj.stenberg at helsinki.fi.invalid
Wed Mar 22 04:18:07 EST 2006

nithya <blueylife at gmail.com> wrote:

> Amanda Gillespie wrote:
>> what have you stained your SDS-PAGE gel with that tells you there is only
>> one band?
>> Amanda

> I have stained it with coomassie blue and i used marker as a known
> protein of same molecular weight.
> Thanking you for any suggestions

1) Coomassie doesn't stain all proteins well. You could try silver
2) Make a symmetric gel, cut in half, stain one half with coomassie or
silver, other half with EtBr.


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