nithya <blueylife at gmail.com> wrote:
> Amanda Gillespie wrote:
>> what have you stained your SDS-PAGE gel with that tells you there is only
>> one band?
>>>> Amanda
>>
> I have stained it with coomassie blue and i used marker as a known
> protein of same molecular weight.
> Thanking you for any suggestions
1) Coomassie doesn't stain all proteins well. You could try silver
staining
2) Make a symmetric gel, cut in half, stain one half with coomassie or
silver, other half with EtBr.
--
Kaj
