[Protein-analysis] Re: Prestained SDS-PAGE markers WITHOUT reducing agent

Allison allison at nospam.com
Sat Mar 18 13:55:49 EST 2006

If you leave a lane free between the markers and your non-reduced 
samples you don't need to remove the reducing agent. I have done this 
many times.

DK wrote:

> In article <mailman.208.1142620927.16885.proteins at net.bio.net>, DA <da8688 at yahoo.com> wrote:
>>Dear all,
>>I am trying to find a company that sells prestained
>>SDS-PAGE markers without added reducing agent.  All of
>>the vendors I have found included reducing agents in
>>their products.  
>>Can someone point me in the right direction?
> Buy whatever prestained markers you like and run 
> them over little spin gel-filtration columns. 
> (Bunch of companies sell them and they are trivial to 
> make out of blue tip, for example). Volume change 
> is minimal and the protein recovery is always >80%. 
> Sephadex G-25 is most common but I like 
> Bio-Rad's P6 better. 
> DK

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