If you leave a lane free between the markers and your non-reduced
samples you don't need to remove the reducing agent. I have done this
many times.
Allison
DK wrote:
> In article <mailman.208.1142620927.16885.proteins at net.bio.net>, DA <da8688 at yahoo.com> wrote:
>>>Dear all,
>>I am trying to find a company that sells prestained
>>SDS-PAGE markers without added reducing agent. All of
>>the vendors I have found included reducing agents in
>>their products.
>>Can someone point me in the right direction?
>>> Buy whatever prestained markers you like and run
> them over little spin gel-filtration columns.
> (Bunch of companies sell them and they are trivial to
> make out of blue tip, for example). Volume change
> is minimal and the protein recovery is always >80%.
>> Sephadex G-25 is most common but I like
> Bio-Rad's P6 better.
>> DK
