I'm trying to detect a 7kDa protein by Western Blot.
I've ran the cell lysate in a 15% acrylamide gel and the problem is,
proteins bellow 14kDa just vanish.
The Molecular standard is broad range (6-150kDa) and even the weight
doesn't show in coomassie or in the ponceau (I've stained half the gel
and transfered the other half).
Does anyone has a tip or similar problem?