[Protein-analysis] DLS at high protein concentration

QCZ heat-capacity at indy.rr.com
Sun Mar 5 21:41:28 EST 2006

Dear all,
I have a simple question on viscosity correction for DLS. In Stokes-Einstein
equation, the viscosity is referred to solvent viscosity. However, I was
told by someone that Rh at high protein concentration should be corrected by
solution viscosity, which is the viscosity of the protein solution.  I do
not think that was right 'cause in that situation only the apparent Rh was
determined and the hydrodynamic non-ideality may exists.  Any comment?

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