[Protein-analysis] re-using hi-trap chelating columns

ferit tüzer feritq at yahoo.com
Wed Jun 14 19:27:42 EST 2006

Do Hi-trap columns charged with nickel and eluted with
300mM imidazole need to be washed of their imidazole
before re-equilibrating with wash buffer(no imidazole)
and loading with same type of protein? 
Thank you

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