Hi there
Is is true that protein purification under denaturing contitions may be
done in room temperature? I'm puryfing my protein from inclusion bodies
using urea solutions and nickel-agarose column. In 4Cdeg urea in my
buffers starts to crystalize! If I perform lysis of the cell in room
temp thus I think all the proteins (also proteases?) are denatured, am
I right? Next step wolud be refolding of the protein in multistep
dialysis, so I will have to make it partially in 20 partially in 4C.
Julio
