SL <nostoc at gmail.com> wrote:
> I am new doing yeast two hyb, and just got some results. My question is,
> how to representing the Y2H result for a manuscript? I saw quite some
> really nice pictures showing either colonies or streaks on plates, and
> some of them have a duplicated one for LacZ assay.
You can buy a device we used to call "frog": It's a metal plate with a
handle that has 48 little metal stamps (~4mm diameter metal rods)
attached to it. These metal stamps fit exactly into the wells of half a
96-well plate. So all you do is dissolve the colonies of interest in
e.g. 100-200ul of water in the 96well plate and stamp them onto a
selection plate. To sterilize the thing just dip it in ethanol and light
it.
I'm afraid I don't know what the correct name of the "frog" is. I would
just call the major lab suppliers and describe the thing to them - they
will figure it out...
The thing looks roughly like this
from the side:
______ Handle
| |
| |
====================== base plate
I I I I I I I I
metal rods
from the bottom:
________________________
| |
| o o o o o o o o |
| |
| o o o o o o o o |
| |
| o o o o o o o o |
| |
| o o o o o o o o |
| |
| o o o o o o o o |
| |
| o o o o o o o o |
|________________________|
cu
Philipp
--
Dr. Philipp Pagel Tel. +49-8161-71 2131
Dept. of Genome Oriented Bioinformatics Fax. +49-8161-71 2186
Technical University of Munich
http://mips.gsf.de/staff/pagel
