[Protein-analysis] Re: Protein purification using mouse anti-Myc Tag IgG (Breslauer)

Lu Falong via proteins%40net.bio.net (by FLLu At genetics.ac.cn)
Sat Dec 23 00:33:52 EST 2006


	For monoclonal antibody, it is essential for you to test whether protein A or protein G works well. You can not predict which one works well though generally protein G works better than protein A do. However, it could be possible that you antibody binds neither protein A nor protein G!

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>   1. Protein purification using mouse anti-Myc Tag IgG (Breslauer)
>Message: 1
>Date: 21 Dec 2006 09:19:03 -0800
>From: "Breslauer" <breslauer1981 At gmail.com>
>Subject: [Protein-analysis] Protein purification using mouse anti-Myc
>	Tag IgG
>To: proteins At net.bio.net
>Message-ID: <1166721543.134327.280240 At a3g2000cwd.googlegroups.com>
>Content-Type: text/plain; charset="iso-8859-2"
>Hi there,
>have question about publications & your experience. I'm going to purify
>partially purifed recombinant protein (but containing ~30% of protein
>contaminations) using affinity chromatography. I produced a lot of
>monoclonal antibody - mouse anti-myc, and my protein has Myc-Tag.
>Saying produced I mean collected supernatant from hybrydoma cells. The
>thing is to purify & bind my antibodies and (it would be great if using
>the same resign/column) pass my protein throuh it, hoping it will bind
>with antibodies. Then wash & elute it. Do u thing that sepharose with
>protein A will be good? Does any of u who have expereince in this
>experiment may discuss this idea of expriment with me? Waiting for your
>Proteins mailing list
>Proteins At net.bio.net
>End of Proteins Digest, Vol 19, Issue 11

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Lu Falong
 Group 808
 Institute of Genetics and Developmental Biology, CAS 
 Beijing, PR China
 FLLu At genetics.ac.cn

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