proteins-request,
For monoclonal antibody, it is essential for you to test whether protein A or protein G works well. You can not predict which one works well though generally protein G works better than protein A do. However, it could be possible that you antibody binds neither protein A nor protein G!
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>>>Today's Topics:
>> 1. Protein purification using mouse anti-Myc Tag IgG (Breslauer)
>>>----------------------------------------------------------------------
>>Message: 1
>Date: 21 Dec 2006 09:19:03 -0800
>From: "Breslauer" <breslauer1981 At gmail.com>
>Subject: [Protein-analysis] Protein purification using mouse anti-Myc
> Tag IgG
>To: proteins At net.bio.net>Message-ID: <1166721543.134327.280240 At a3g2000cwd.googlegroups.com>
>Content-Type: text/plain; charset="iso-8859-2"
>>>Hi there,
>>have question about publications & your experience. I'm going to purify
>partially purifed recombinant protein (but containing ~30% of protein
>contaminations) using affinity chromatography. I produced a lot of
>monoclonal antibody - mouse anti-myc, and my protein has Myc-Tag.
>Saying produced I mean collected supernatant from hybrydoma cells. The
>thing is to purify & bind my antibodies and (it would be great if using
>the same resign/column) pass my protein throuh it, hoping it will bind
>with antibodies. Then wash & elute it. Do u thing that sepharose with
>protein A will be good? Does any of u who have expereince in this
>experiment may discuss this idea of expriment with me? Waiting for your
>replay.
>>Bres
>>>>------------------------------
>>_______________________________________________
>Proteins mailing list
>Proteins At net.bio.net>http://www.bio.net/biomail/listinfo/proteins>>End of Proteins Digest, Vol 19, Issue 11
>****************************************
>
= = = = = = = = = = = = = = = = = = = =
Sincerely,¡¡¡¡¡¡¡¡¡¡
Lu Falong
Group 808
Institute of Genetics and Developmental Biology, CAS
Beijing, PR China
FLLu At genetics.ac.cn
2006-12-23
