Ad Polytron homogeniser Kinematica
I used this apparatous already, but never disassembled it. I only released
the generator by pushing a buttom and turning the generator. Before removing
it, we used to clean the generator by running the homogenizer in a 10 % SDS
solution, followed by running it in a 70% ethanol solution. Because of aerosol,
I placed the homogeniser into a fume hood to run it. The homogeniser can
be autoclaved as described on the company?s homepage under FAQ: http://www.brinkmann.com/faqs/Homogenizers.asp
Sincerely
Dr Nicole MR Schmitz
>-- Originalnachricht --
>Date: Tue, 12 Dec 2006 12:01:16 -0500 (EST)
>From: proteins-request At oat.bio.indiana.edu>Subject: Proteins Digest, Vol 19, Issue 6
>To: proteins At magpie.bio.indiana.edu>Reply-To: proteins At oat.bio.indiana.edu>>>Send Proteins mailing list submissions to
>proteins At net.bio.net>>To subscribe or unsubscribe via the World Wide Web, visit
>http://www.bio.net/biomail/listinfo/proteins>or, via email, send a message with subject or body 'help' to
>proteins-request At net.bio.net>>You can reach the person managing the list at
>proteins-owner At net.bio.net>>When replying, please edit your Subject line so it is more specific
>than "Re: Contents of Proteins digest..."
>>>Today's Topics:
>> 1. Dismantling Polytron EasyCare Homogeniser (IanMc)
> 2. Re: protease (ChenHA)
>>>----------------------------------------------------------------------
>>Message: 1
>Date: Mon, 11 Dec 2006 13:10:08 -0000
>From: "IanMc" <zebedeeboy At hotmail.com>
>Subject: [Protein-analysis] Dismantling Polytron EasyCare Homogeniser
>To: proteins At net.bio.net>Message-ID: <mailman.390.1165873983.19683.arrays At net.bio.net>
>>Hi,
>>I am getting nowhere fast with the companies who sold me our Polytron
>homogeniser. Does anyone know how to take apart a Kinematica/Brinkman
>Polytron homegeniser generator for cleaning? We are using it on gut samples
>>and sheets of connective tissue are getting trapped between the rotor and
>>stator. They ship a 'tool' with the generator but without any instructions
>>on how to use it.
>>Anybody out there who has overcome this particular problem?
>>TIA,
>>Ian Mc
>>>>>------------------------------
>>Message: 2
>Date: Mon, 11 Dec 2006 22:26:06 +0000
>From: ChenHA <hzhen At freeuk.com>
>Subject: [Protein-analysis] Re: protease
>To: proteins At net.bio.net>Message-ID: <qglrn2h3vg0jnpquthmuhm952tdnnn89ig At 4ax.com>
>Content-Type: text/plain; charset=us-ascii
>>On Mon, 11 Dec 2006 16:20:49 +0100, Abla Sipanis
><ablasipanis At yahoo.co.uk> wrote:
>>>Hi all,
>>>>I'm looking for a protease that has a very specific cleavage site, and
>>quite long so that it wouldn't cut at all in the whole Arabidopsis
>>proteome. Do you think that this does exist ?
>>Amersham for example claims that the recognition site for Prescission is
>>>LeuGluValLeuPheGln/GlyPro. Does it mean that this is really the only
>>sequence that will be cut ? And by the way, I would like to express this
>>>protease in vivo, so that I would need a cDNA. Perhaps difficult for a
>>patented enzyme ?
>>I very much doubt that it would cut only at that specific sequence.
>You can check the literature. Prescission is actually 3C protease
>from human rhinovirus, so have a look. If I remember correctly, it
>can cleave a number of different sequences.
>>We make our own 3C protease from HRV 14 although I'm not sure if it is
>the exact same one as Prescission (not really sure if there are
>different kinds of 3C proteases from HRV, have a look in the
>literature). The one we use belongs to another lab, so can't offer it
>to you myself, but it's something you can clone it yourself. It is
>fused to GST in pGEX and needs to be expressed at low temperature (18
>to 20 deg C) or else it would form inclusion body.
>>>>>>>>Any suggestion would be ighly appreciated.
>>Thanks for you help !
>>>>Abla
>>>>------------------------------
>>_______________________________________________
>Proteins mailing list
>Proteins At net.bio.net>http://www.bio.net/biomail/listinfo/proteins>>End of Proteins Digest, Vol 19, Issue 6
>***************************************
Neu: Das erste ADSL-Abo ohne Monatsgebühr! Steigen Sie jetzt auf sunrise
ADSL free um.
http://www.sunrise.ch/privatkunden/iminternetsurfen/adsl/adsl_abosundpreise/adsl_gelegenheitssurfer/adsl_free.htm
