[Protein-analysis] Re: regarding immunoprecipitation

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum At hotmail.com)
Sat Dec 2 05:03:13 EST 2006

Ramachandran Balasubramanian wrote:

> hello everyone,
> I have been working on immunoprecipitation ie pull-down of my protein
> using a monoclonal antibody 
> Can anyone suggest a good procedure for pull-down assays. or is my protein
> interaction has some problem.

Calbiochem sells Pansorbin, killed Staph. aureus cells expressing
protein A on their surface. They also make a nice booklet on its use
with lots of recipes. Handy even if you prefere protein A agarose over

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