Ramachandran Balasubramanian wrote:
> hello everyone,
>> I have been working on immunoprecipitation ie pull-down of my protein
> using a monoclonal antibody
>> Can anyone suggest a good procedure for pull-down assays. or is my protein
> interaction has some problem.
Calbiochem sells Pansorbin, killed Staph. aureus cells expressing
protein A on their surface. They also make a nice booklet on its use
with lots of recipes. Handy even if you prefere protein A agarose over
Pansorbin.