[Protein-analysis] Ammonium bicarbonate in IE chromatography

[pawel.stocki at ncl.ac.uk]

Hi,
Does anyone have some experience in using ammonium bicarbonate buffer
for gradient elution from Q-Sepharose or other IE? I would like to use
it instead of NaCl gradient. The sample will be subjected for further
MS analysis that is why I would like to use volatile salt. I'll
appreciate any comments. 

Thanks,
Pawel