Dr Engelbert Buxbaum wrote:
> I am not sure why you included the Tris in the first place, imidazole is
> a perfectly good buffer.
It wasn't completely by choice -- all the buffers for the Novagen
His-bind kit are Tris based. It would not be a problem to make them
without Tris, but obviously I was not anticipating this problem with
the dialysis.
