Enzyme immobilization.

Chris B. chris.b.nospam at nospam.interia.pl
Tue May 17 04:01:10 EST 2005

This may be stupid and/or simple question but I can't find answer...
Can I and how I can bind protease (pepsin, trypsin) to chitosan or sephadex?
I heard about binding enzyme to chitosan using glutaraldehyde and to
sepharose using cyanogen bromide.
Does anybody have good and cheap method to share with protein science
freshman? :)

And one more thing - what is stability of such an enzyme? After how much
time or how many use it looses activity (below "resonable" level)?

Thanks in advance...


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