IUBio

Immunoprecipitation without beads?

Ben Long long at rsbs.anu.edu.au
Thu Mar 10 01:03:20 EST 2005


Hi all,

I am looking for an immunoprecipitation protocol where I can precipitate
antigen/antibody complexes with just protein A (i.e. not bound to sepharose
or agarose beads).  The only reason I want to do this is simply that I have
some protein A available to me at the moment but not protein A beads.  If
this can be done and anyone has a protocol I would love to know what to do.

Thanks in advance,

Ben
ben.long at anu.edu.au





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