> Hi All
>> For western blot, is it possible to separate proteins of size 66kDa and
> 70kDa using gradient gel? What percentage of gel should be used?
I have done something like that once with Hsc70 on a 5-10% gradient gel.
You may also whish to check for the use of Na-tetradecylsulphate as
detergent, which allowed the separation of Na/K-ATPase isoforms that
could not be separated in the presence of SDS.