Bradford Protein Assay in membrane crystals

Gerd Hoffmann gerd.hoffmann at nambition.com
Wed Jan 19 09:44:34 EST 2005


we want to try to determine protein concentration in a solution
containing fragments of "membrane-protein-crystalls". We
want to try this with a bradford assay.
Unfortunately these fragments are about 1 micron in diameter
and therefore to big for a simple photometric determination.
So we have to get the proteins out of the membrane. We can
do this for example by using detergents. So here are my
questions: Is there any inteference between the bradford assay
and the detergent? And if so, are there other possibilities
to measure the concentration of the protein in our solution?

Thank you for your ansewers!


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