IUBio

DDT vs. 2-ME?

D.K. noemail at thankstospam.edu
Wed Feb 2 17:58:52 EST 2005


In article <BE26CBB8.25CE%nikolai.scherbak at nat.oru.se>, nikolai.scherbak at nat.oru.se (Nikolai Scherbak) wrote:
>Dear colleagues,
>
>Is it any difference in using DTT vs. 2-ME in loading buffer for SDS-PAGE
>under reducing conditions ?

Yes, DTT is a better reducer so results are cleaner - particularly 
when sample boiling needs to be limited/eliminated. 

DK



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