nithya wrote:
> I have done a trypsin digestion of a coloured protein.........
> my protocol is 1mg/ml trypsin in 0.1% acetic acid.......
> protein soln 40 ug/ml in 100 mM AmBic buffer ph:8.5
> overnight incubation
> prot: trypsin ratio : 100:1/ 50:1
>> my boss says that i need to c a reduction in color of the prot if
> digestion has occurred.....
>> is ther any other way to confirm that digestion has occurred
> completely?
>> I would welcome any suggestions on the protocol as well
>> have to do MS of the digested peptides
>> help me
>> thanks
>> Nithya
>Run an SDS-PAGE gel--does your full-length protein disappear? You'll
have to run a gel before MS anyway.
