I have done a trypsin digestion of a coloured protein.........
my protocol is 1mg/ml trypsin in 0.1% acetic acid.......
protein soln 40 ug/ml in 100 mM AmBic buffer ph:8.5
overnight incubation
prot: trypsin ratio : 100:1/ 50:1
my boss says that i need to c a reduction in color of the prot if
digestion has occurred.....
is ther any other way to confirm that digestion has occurred
completely?
I would welcome any suggestions on the protocol as well
have to do MS of the digested peptides
help me
thanks
Nithya
