His tagged protein won't bind

Dr. Barry Panaretou barry.panaretou at kcl.ac.uk
Tue Sep 14 12:45:25 EST 2004

We've tagged a protein with 6xHis, and expressed it in E.coli. It didn't
bind nickel resin. We also tagged the C -terminus and again it didn't
bond to nickel resin. Has anyone come across this before (protein is in
supernatant after hard spin, so seems soluble--can also detrect with
anti-his antobody on westerns, in both cases)



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