We've tagged a protein with 6xHis, and expressed it in E.coli. It didn't
bind nickel resin. We also tagged the C -terminus and again it didn't
bond to nickel resin. Has anyone come across this before (protein is in
supernatant after hard spin, so seems soluble--can also detrect with
anti-his antobody on westerns, in both cases)
Barry
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