Exposing your western blot to X-ray film? Are you doing 35S labeling of
proteins or immunodetection? Sulphur-35 emmissions will not go through
saran/plastic wraps.
Quoting tunugus at mail.rockefeller.edu:
>> hi
>> is there a differnece between saran wrap & fischer brand all purpose wrap? i
> have been using the latter for western blot to wrap my membrane before
> exposing my film.
>> I am also suspecting this might be a cause for not getting any bands on my
> film.
> I am not sure if the wrap can make a difference.
>> Any inputs on ths one,please help.
> Sari
>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0>
--
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
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