josekolich at yahoo.com wrote:
>> Can you help me with this?
>> 1. What are the main factor affecting a photometrci enzime determination
> and what are the main sources of error?
>> 2. What relationship exist between Km and substrate concentration as a
> enzimatically catalized reaction reach an 80% of Vmax.
>> Thank you
Trying to let us do your homework?
Do a little reading on your own. For question one, the keyword is
"Warburg's coupled spectrophotometric assay", for the second one