two inquiries about enzimes. Can you help me?

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Tue May 11 00:25:42 EST 2004

josekolich at yahoo.com wrote:

> Can you help me with this?
> 1. What are the main factor affecting a photometrci enzime determination
> and what are the main sources of error?
> 2. What relationship exist between Km and substrate concentration as a
> enzimatically catalized reaction reach  an 80% of Vmax.
> Thank you
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0

Trying to let us do your homework?

Do a little reading on your own. For question one, the keyword is
"Warburg's coupled spectrophotometric assay", for the second one
"Michaelis-Menten kinetics"

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